RIPA (RadioImmunoPrecipitation Assay) lysis buffer1% (w/w) Nonidet P-40 (NP-40)1% (w/v) sodium deoxycholate.0.1% (w/v) SDS.0.15 M NaCl.0.01 M sodium phosphate, pH 7.2.2 mM EDTA.50 mM sodium fluoride (NaF)0.2 mM fresh sodium orthovanadate (Na3VO4.2H2O, it has phosphatase inhibitor function because it mimics phosphate)More items...read more >>